Custom acquisition, analysis and processing plugins can be developed using ImageJ’s built in editor and Java compiler. ImageJ was designed with an open architecture that provides extensibility via Java plugins. Density or gray scale calibration is also available. Spatial calibration is available to provide real world dimensional measurements in units such as millimeters. The program supports any number of windows (images) simultaneously, limited only by available memory. All analysis and processing functions are available at any magnification factor. Image can be zoomed up to 32 : 1 and down to 1 : 32. It does geometric transformations such as scaling, rotation and flips. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering. It can create density histograms and line profile plots. It can calculate area and pixel value statistics of user-defined selections. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations A somehow outdated list of ImageJ’s features is available at. It supports ‘stacks’ (and hyperstacks), a series of images that share a single window. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and ‘raw’. It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. Downloadable distributions are available for Windows, Mac OS X and Linux. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.5 or later virtual machine. ImageJ is a public domain Java image processing and analysis program inspired by NIH Image for the Macintosh. 3.1.1 Ethics in Scientific Image Processing.2.2.1 Software Packages Built on Top of ImageJ.
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